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Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis


Citation

Yuli, Haryani and Awang Saleh, Noorzaleha and Abu Bakar, Fatimah and Mohamed Alitheen, Noorjahan Banu and Patrick, Guda Benjamin and Abdul Talib, Shamsinar and Ahmad Suhaimi, Laila Rabaah and Radu, Son (2007) Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis. Food Control, 18 (7). pp. 847-853. ISSN 0956-7135

Abstract / Synopsis

A total of 78 samples of street foods from different states in Malaysia were examined for the presence of Klebsiella pneumonia. K. pneumonia contamination was recorded in 32% of the samples examined. The frequency of the K. pneumonia isolates containing plasmids was shown to be 48%. Susceptibility testing showed that all isolates were highly resistant to ampicillin (100%), erythromycin (100%), rifampicin (100%), streptomycin (96%) and sulfamethoxazole (80%), but susceptible to chloramphenicol. RAPD proved to be the most effective technique in discriminating the K. pneumonia isolates since dendrogram constructed using the combination of 4 primers could differentiate 25 isolates of K. pneumonia to 25 strains. This is the first report that revealed the occurrence of K. pneumonia in street foods sold in Malaysia.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
DOI Number: 10.1016/j.foodcont.2006.04.009
Publisher: Elsevier
Keywords: Street foods, Klebsiella pneumonia, Plasmid, Antibiotic resistance, RAPD, PCR
Depositing User: Samsida Samsudin
Date Deposited: 10 Jun 2010 11:09
Last Modified: 11 Nov 2015 11:06
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodcont.2006.04.009
URI: http://psasir.upm.edu.my/id/eprint/7155
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