Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay
Lee, Thong Chuan and Yusoff, Khatijah and Nathan, Sheila and Tan, Wen Siang (2006) Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay. Journal of Virological Methods, 136 (1-2). pp. 224-229. ISSN 0166-0934
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Official URL: http://dx.doi.org/10.1016/j.jviromet.2006.05.017
Newcastle disease virus (NDV) strains can be classified as virulent or avirulent based upon the severity of the disease. Differentiation of the virus into virulent and avirulent is necessary for effective control of the disease. Biopanning experiments were performed using a disulfide constrained phage displayed heptapeptide library against three pathotypes of NDV strains: velogenic (highly virulent), mesogenic (moderately virulent) and lentogenic (avirulent). A phage clone bearing the peptide sequence SWGEYDM capable of distinguishing virulent from avirulent NDV strains was isolated. This phage clone was employed as a diagnostic reagent in a dot blot assay and it successfully detected only virulent NDV strains.
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