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Nutritional factors affecting organic solvent-tolerant alkaline protease production by a new Bacillus cereus strain 146


Citation

Shafee, Norazizah and Tan, Chin Chin and Mahamad, Shalihah and Raja Abdul Rahman, Raja Noor Zaliha and Basri, Mahiran and Salleh, Abu Bakar (2006) Nutritional factors affecting organic solvent-tolerant alkaline protease production by a new Bacillus cereus strain 146. Annals of Microbiology, 56 (1). pp. 29-34. ISSN 1590-4261; ESSN: 1869-2044

Abstract / Synopsis

An organic solvent-tolerant bacterium designated as 146 capable of producing an organic solvent-stable alkaline protease was isolated from contaminated soil of a wood factory. The strain was a Gram-positive, spore-forming, nitrate-positive, rod-shaped organism capable of hydrolysing gelatine, starch, skim milk and identified as Bacillus cereus. Activity of the protease was drastically increased in the presence of 1–decanol, isooctane, n-dodecane and n-tetradecane, but reduced in the presence of ethyl acetate, benzene, toluene, 1-heptanol, ethylbenzene and hexane. The bacterium was shown to require lactose as a carbon source and peptone as a nitrogen source. The optimum fermentation condition for the production of alkaline protease was in the presence of beef and yeast extract. Optimum pH was determined to be at 10.0 at incubation temperature of 37 °C for 48 h. Results from the studies suggest that 146 is a new strain of Bacillus cereus capable of producing organic solvent-tolerant alkaline protease with potential use in industries.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
DOI Number: 10.1007/BF03174966
Publisher: Springer
Keywords: Organic solvent-tolerant; Alkaline protease; Bacillus cereus; Media composition
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 09 Jun 2010 11:02
Last Modified: 26 Sep 2016 16:37
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/BF03174966
URI: http://psasir.upm.edu.my/id/eprint/7109
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