Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester
Gunawan, Erin Ryantin, Basri, Mahiran, Abdul Rahman, Mohd Basyaruddin, Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha (2005) Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester. Enzyme and Microbial Technology, 37 (7). pp. 739-744. ISSN 0141-0229
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Official URL: http://dx.doi.org/10.1016/j.enzmictec.2005.04.010
The synthesis of wax ester using refined, bleached and deodorized (RBD) palm oil and oleyl alcohol catalyzed by lipozyme IM was carried out. Response surface methodology (RSM) based on a five-level, four-variable central composite rotatable design (CCRD) was used to evaluate the interactive effects of synthesis, of reaction time (2.5–10 h), temperature (30–70 °C), amount of enzyme (0.1–0.2 g) and substrate molar ratio (palm oil to oleyl alcohol, 1:1–1:5) on the percentage yield of wax esters. The optimum conditions derived via RSM were: reaction time 7.38 h, temperature 53.9 °C, amount of enzyme 0.149 g, and substrate molar ratio 1:3.41. The actual experimental yield was 84.6% under optimum condition, which compared well to the maximum predicted value of 85.4%.
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