Gunawan, Erin Ryantin and Basri, Mahiran and Abdul Rahman, Mohd Basyaruddin and Salleh, Abu Bakar and Raja Abdul Rahman, Raja Noor Zaliha (2005) Study on response surface methodology (RSM) of lipase-catalyzed synthesis of palm-based wax ester. Enzyme and Microbial Technology, 37 (7). pp. 739-744. ISSN 0141-0229
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Official URL: http://dx.doi.org/10.1016/j.enzmictec.2005.04.010
The synthesis of wax ester using refined, bleached and deodorized (RBD) palm oil and oleyl alcohol catalyzed by lipozyme IM was carried out. Response surface methodology (RSM) based on a five-level, four-variable central composite rotatable design (CCRD) was used to evaluate the interactive effects of synthesis, of reaction time (2.5–10 h), temperature (30–70 °C), amount of enzyme (0.1–0.2 g) and substrate molar ratio (palm oil to oleyl alcohol, 1:1–1:5) on the percentage yield of wax esters. The optimum conditions derived via RSM were: reaction time 7.38 h, temperature 53.9 °C, amount of enzyme 0.149 g, and substrate molar ratio 1:3.41. The actual experimental yield was 84.6% under optimum condition, which compared well to the maximum predicted value of 85.4%.
|Keyword:||Response surface methodology (RSM), Central composite rotatable design (CCRD), Palm oil, Lipozyme, Alcoholysis, Wax ester|
|Faculty or Institute:||Faculty of Science|
|Deposited By:||Erni Suraya Abdul Aziz|
|Deposited On:||09 Jun 2010 03:12|
|Last Modified:||27 Jul 2010 09:18|
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