An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: Nutritional factors affecting protease production

Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Basri, Mahiran and Salleh, Abu Bakar (2005) An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: Nutritional factors affecting protease production. Enzyme and Microbial Technology, 36 (5-6). pp. 749-757. ISSN 0141-0229

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Official URL: http://dx.doi.org/10.1016/j.enzmictec.2004.12.022

Abstract

An organic solvent-tolerant bacterium producing an organic solvent-stable protease was isolated from soil and identified as Pseudomonas aeruginosa strain K. Nutritional requirements for optimized protease production by this strain were investigated. Maximum protease activity was achieved with sorbitol as the sole carbon source, followed by starch and lactose at pH 7.0 and 37 °C. Dextrose, sucrose and glycerol greatly reduced the protease production. The best organic nitrogen source was casamino acid. Tryptone, soytone and yeast extract supported protease production while corn steep liquor and beef extract inhibited the protease activity. Significant protease production was observed with sodium nitrate as a sole nitrogen source however, ammonium nitrate completely inhibit it. More than 62% drop in production occurred in the presence of amino acids. Addition of metal ions such as K+, Mg2+ and Ca2+ maximized the enzyme production.

Item Type:Article
Keyword:Pseudomonas, Organic solvent-tolerant, Lipase, Optimization
Faculty or Institute:Faculty of Environmental Studies
DOI Number:10.1016/j.enzmictec.2004.12.022
ID Code:7058
Deposited By: Erni Suraya Abdul Aziz
Deposited On:04 Jun 2010 06:45
Last Modified:27 Jul 2010 08:55

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