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An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production

Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Basri, Mahiran and Salleh, Abu Bakar (2005) An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: nutritional factors affecting protease production. Enzyme and Microbial Technology, 36 (5-6). pp. 749-757. ISSN 0141-0229; ESSN: 1879-0909

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Abstract / Synopsis

An organic solvent-tolerant bacterium producing an organic solvent-stable protease was isolated from soil and identified as Pseudomonas aeruginosa strain K. Nutritional requirements for optimized protease production by this strain were investigated. Maximum protease activity was achieved with sorbitol as the sole carbon source, followed by starch and lactose at pH 7.0 and 37 °C. Dextrose, sucrose and glycerol greatly reduced the protease production. The best organic nitrogen source was casamino acid. Tryptone, soytone and yeast extract supported protease production while corn steep liquor and beef extract inhibited the protease activity. Significant protease production was observed with sodium nitrate as a sole nitrogen source however, ammonium nitrate completely inhibit it. More than 62% drop in production occurred in the presence of amino acids. Addition of metal ions such as K+, Mg2+ and Ca2+ maximized the enzyme production.

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
DOI Number: 10.1016/j.enzmictec.2004.12.022
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.enzmictec.2004.12.022
Publisher: Elsevier
Keywords: Pseudomonas; Organic solvent-tolerant; Lipase; Optimization
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 04 Jun 2010 14:45
Last Modified: 26 Sep 2016 16:46
URI: http://psasir.upm.edu.my/id/eprint/7058

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