Large Scale Production of Liquid Wax Ester by Immobilized Lipase
Mat Radzi, Salina, Basri, Mahiran, Salleh, Abu Bakar, Ariff, Arbakariya, Mohammad, Rosfarizan and Abdul Rahman, Mohd Basyaruddin (2005) Large Scale Production of Liquid Wax Ester by Immobilized Lipase. Journal of Oleo Science, 54 (4). pp. 203-209. ISSN 1347-3352
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Official URL: http://www.jstage.jst.go.jp/article/jos/54/4/54_20...
Oleyl oleate, a liquid wax ester was synthesized by an immobilized Candida antartica lipase B (Novozym 435) as biocatalyst using oleic acid and oleyl alcohol. The effects of various reaction parameters were optimized to obtain a high yield of liquid wax esters. Investigation in large scale production was performed in batch mode of stirred tank reactor (STR) with one multi-bladed impeller. The optimum condition to produce liquid wax ester was, reaction time (RT); 30 min, temperature (T); 50 °C, amount of enzyme (E); 90 g (900,000 PLU), agitation speed (A) of 400 rpm, number of impeller tip (N) of 2 and molar ratio of oleyl alcohol to oleic acid (M); 2:1. Analysis of the yield showed that at optimum condition, >90% liquid wax esters were produced. The stability of Novozym 435 showed at high percentage (>80%) up to 4 cycles.
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