Identification of Epitopes In The Nucleocapsid Protein of Nipah Virus Using a Linear Phage-Displayed Random Peptide Library

Eshaghi, Majid and Wen, Siang Tan and Yusoff, Khatijah (2005) Identification of Epitopes In The Nucleocapsid Protein of Nipah Virus Using a Linear Phage-Displayed Random Peptide Library. Journal of Medical Virology, 75 (1). pp. 147-152. ISSN 0146-6615

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Official URL: http://dx.doi.org/10.1002/jmv.20249

Abstract

A random peptide library of heptamers displayed on he surface of M13 bacteriophage was used to identify specific epitopes of antibodies in pooled sera of swine naturally infected by Nipah virus. The selected heptapeptides were aligned with proteinsequences of Nipah virus and several putative epitopes were indentified within the nucleocapsid protein. A total of 41 to 60 (68%) selected phage clones had inserts resembling a region with the sequence SNRTQGE, located at the C-terminal end (amino acids 503-509) of the nucleocapsid protein. The binding of antibodies in the swine and human antisera to the phage clone was inhibited by synthetic peptide corresponding to this region. Epitopes identified by phage display are consistent with the predicted antigenic sites for the Nipah virus nucleocapsid protein. The selected phage clone used as a coating antigen discriminated swine and human Nipah virus sera-positive from sera-negative samples exhibit characteristics, which might be attractive for diagnostic tests.

Item Type:Article
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:Wiley-Blackwell
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1002/jmv.20249
ID Code:704
Deposited By:INVALID USER
Deposited On:24 Nov 2008 19:13
Last Modified:30 Jul 2010 04:09

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