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Regions on Nucleocapsid Protein of Newcastle Disease Virus that Interact with its Phosphoprotein


Citation

Chiew, Ling Kho and Tan, Wen Siang and Tey, Beng Ti and Yusoff, Khatijah (2004) Regions on Nucleocapsid Protein of Newcastle Disease Virus that Interact with its Phosphoprotein. Archives of Virology, 149 (1). pp. 997-1005. ISSN 0304-8608

Abstract / Synopsis

Summary. The nucloecapsid (NP) and phosphor-(P) proteins of paramyxoviruses are involved in transcription and replication of the viral genome. An in vitro protein binding assay was used to investigate the regions on NP protein that interact with the P protein of Newcastle disease virus (NDV). Truncated NP mutants were first immobilized on a solid phase and then interacted with radio-labelled [35S]-P protein synthesised in rabbit reticulocyte. The interaction affinity was quantitated by measuring the radioactivity that was retained on the solid phase. Using the approach, a highly interactive region was identified to be resided at the first 25 amino acids of NPN-terminal. The interaction between these two proteins remained strong even with the removal of 114 amino acids from the C-terminal end might have another contact region for P protein, which is not as critical as the N_terminal end. The interaction regions mapped in this study are significantly different from the other two paramyxoviruses: Sendai and measles viruses in which the C-termini of their NP proteins play an important role in binding to the P.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Springer Wien
Depositing User: Users 17 not found.
Date Deposited: 25 Nov 2008 00:07
Last Modified: 27 Nov 2015 10:06
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s00705-003-0273-8
URI: http://psasir.upm.edu.my/id/eprint/695
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