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Determination of Daidzein and Genistein Contents in Mangifera Fruit.


Citation

Khoo, Hock Eng and Ahmad, Ismail (2008) Determination of Daidzein and Genistein Contents in Mangifera Fruit. Malaysian Journal of Nutrition, 14 (2). pp. 189-198. ISSN 1394-035X

Abstract / Synopsis

The aim of this study was to determine the daidzein and genistein contents in Mangifera fruits. Three Mangifera species namely ‘bacang’ (Mangifera foetida), ‘kuini’ (M. odorata) and ‘bambangan’ (M. pajang) each from two different locations were selected. The extraction of isoflavones was carried out at 80oC for 30, 60 and 90 min. HPLC method was performed with a flow rate of 1.00 ml/min using three different separation columns to determine isoflavone contents. The Zorbax Eclipse RP C18 reverse-phase column was found to give the best resolution for isoflavone separation in Mangifera fruits. Moreover, extraction time of 90 min was found to increase the isoflavone aglycone contents. At optimised condition, ‘kuini’ had relatively high daidzein (9.4-10.5 mg/100 g) and genistein (1.6-1.7 mg/100 g) contents. Daidzein content of ‘bambangan’ (8.3-8.7 mg/100 g) was higher than ‘bacang’, but the genistein content of ‘bambangan’ (0.4-0.6 mg/100 g) was similar to that of ‘bacang’ (0.4-0.8 mg/100 g). However, there was a variation in daidzein and genistein contents in Mangifera fruits between two geographical locations.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Publisher: Nutrition Society of Malaysia
Depositing User: Anas Yahaya
Date Deposited: 18 May 2010 11:11
Last Modified: 18 Sep 2014 16:54
URI: http://psasir.upm.edu.my/id/eprint/6495
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