Determination of the Presence of Huanglongbing in Seeds and Movement of the Pathogen in Citrus reticulata

Shokrollah, Hajivand and Abdullah, Thohirah Lee and Sijam, Kamaruzaman and Abdullah, Siti Nor Akmar (2009) Determination of the Presence of Huanglongbing in Seeds and Movement of the Pathogen in Citrus reticulata. American Journal of Applied Sciences, 6 (6). pp. 1180-1185. ISSN 1546-9239

Full text not available from this repository.

Official URL:


Problem statement: Huanglongbing (HLB) also known as citrus greening disease is a fastidious phloem-inhabiting bacterium in the genus Candidatus Liberibacter. Using universal primers, the 16S ribosomal DNA sequence of three strains of the bacterium were obtained by PCR. However there is very little information on seed transmission and HLB pathogen movement to find a way for control or reduce the severity of HLB on the field. The study was conducted to detect HLB pathogen in seeds of Citrus, to determine pathogen movement in citrus seedling after infection and to detect the HLB pathogen in citrus roots. Approach: Seeds of Citrus reticulata cv. Limau Madu were collected from infected orchard and were germinated in screenhouse condition. The seeds of Citrus reticlata cv. L. Madu were planted in screenhouse too for HLB pathogen movement and HLB detection in roots. The seedlings were inoculated using infected grafting methods. Results: HLB was not amplified in new seedlings after germination. HLB moved slowly reaching up to 1.5 cm after 2 weeks, 1.5-4.5 cm after eight weeks and detected on 4.5-9 cm after 14 weeks below the grafting area. HLB was also detected up to 9-15 cm after 16 weeks, 15-24 cm after twenty weeks, 24-28.5 cm after 22 weeks and 28.5-30 cm after 24 weeks below the grafting area. Conclusion: Base on conventional PCR test, HLB disease in citrus is not seed borne and it can reach to the roots 26 weeks after inoculation.

Item Type:Article
Keyword:HLB, seed borne, pathogen movement, greening disease, citrus
Faculty or Institute:Faculty of Agriculture
ID Code:6319
Deposited By: Norhazura Hamzah
Deposited On:13 May 2010 02:07
Last Modified:22 Jul 2010 04:54

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 13 May 2010 02:07.

View statistics for "Determination of the Presence of Huanglongbing in Seeds and Movement of the Pathogen in Citrus reticulata"

Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.