Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep

Mohd Yusoff, Md Sabri and Saad, Mohd Zamri and Mutalib, Abdul Rahim and Israf Ali, Daud Ahmad and N., Muniandy (2000) Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep. Veterinary Microbiology, 73 . pp. 13-23. ISSN 0378-1135

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Official URL: http://dx.doi.org/10.1016/S0378-1135(99)00205-9

Abstract

The outer membrane proteins (OMP) were extracted from the P. haemolytica A2, A7 and A9 to determine their potential as immunogens and their capability for cross-protection. Sixty lambs of approximately 9 months old were divided into four main groups. Animals in Group 1 were vaccinated with 2 ml vaccine containing 100 mg/ml of the outer membrane proteins of P. haemolytica A2. Animals in Group 2 were similarly vaccinated with the OMPs of P. haemolytica A7 while Group 3 with OMPs of P. haemolytica A9. Animals in Group 4 were unvaccinated control. During the course of the study, serum was collected to evaluate the antibody levels toward each OMP. There appeared to be good immune responses. However, high antibody levels did not necessarily result in good protection of the animals, particularly against cross-infection with P. haemolytica A9 in animals vaccinated with the OMPs of P. haemolytica A2. It seemed that the antibody responses were more speci®c toward the homologous challenge but generally did not cross-protect against heterologous serotype challenge. However, the OMPs of P. haemolytica A7 produced good in vivo cross-protection and excellent correlations when good antibody responses against all serotypes led to successful reductions of the extent of lung lesions following homologous and heterologous challenge exposures. Thus, the OMPs of P. haemolytica A7 was effective in protecting animals against homologous and heterologous infection by live P. haemolytica A2, A7 and A9.

Item Type:Article
Keyword:Outer membrane proteins, Pasteurella haemolytica, Vaccine, Sheep
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:2000 Elsevier Science B.V.
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/S0378-1135(99)00205-9
ID Code:6281
Deposited By: Azizan Arshad
Deposited On:12 May 2010 07:24
Last Modified:26 Jul 2010 09:54

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