Citation
Abstract
Optically active (−)-menthyl butyrate was synthesized by enantioselective esterification of racemic (±)-menthol and butyric anhydride using lipase from Candida rugosa immobilized onto epoxy-activated supports of Eupergit C and Eupergit C 250 L through physical adsorption method. The effects of various temperature, storage condition, stability in organic solvent and lipase recyclability were investigated for their influence on the enzymatic enantioselective formation of (−)-menthyl butyrate. The immobilized lipases retained high catalytic activity of up to 31% yield and 100% enantiomeric excess of the desired product, and showed better stability compared to the native lipase. They also exhibited about 50% retained activity even after incubation at higher temperatures, storage at room temperature and after long incubation in hexane. Immobilized lipases also showed considerably efficient reusability.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Science Faculty of Biotechnology and Biomolecular Sciences |
| DOI Number: | https://doi.org/10.1016/j.foodchem.2007.04.026 |
| Publisher: | Elsevier |
| Keywords: | Immobilization; Lipase; Enantioselectivity; Epoxide; Menthyl butyrate |
| Depositing User: | Najwani Amir Sariffudin |
| Date Deposited: | 12 May 2010 08:54 |
| Last Modified: | 28 Sep 2016 04:05 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodchem.2007.04.026 |
| URI: | http://psasir.upm.edu.my/id/eprint/6144 |
| Statistic Details: | View Download Statistic |
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