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Production of highly enantioselective (−)-menthyl butyrate using Candida rugosa lipase immobilized on epoxy-activated supports


Citation

Othman, Siti Salhah and Basri, Mahiran and Hussein, Mohd Zobir and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Jasmani, Halila (2008) Production of highly enantioselective (−)-menthyl butyrate using Candida rugosa lipase immobilized on epoxy-activated supports. Food Chemistry, 106 (2). pp. 437-443. ISSN 0308-8146; ESSN: 1873-7072

Abstract / Synopsis

Optically active (−)-menthyl butyrate was synthesized by enantioselective esterification of racemic (±)-menthol and butyric anhydride using lipase from Candida rugosa immobilized onto epoxy-activated supports of Eupergit C and Eupergit C 250 L through physical adsorption method. The effects of various temperature, storage condition, stability in organic solvent and lipase recyclability were investigated for their influence on the enzymatic enantioselective formation of (−)-menthyl butyrate. The immobilized lipases retained high catalytic activity of up to 31% yield and 100% enantiomeric excess of the desired product, and showed better stability compared to the native lipase. They also exhibited about 50% retained activity even after incubation at higher temperatures, storage at room temperature and after long incubation in hexane. Immobilized lipases also showed considerably efficient reusability.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Faculty of Biotechnology and Biomolecular Sciences
DOI Number: 10.1016/j.foodchem.2007.04.026
Publisher: Elsevier
Keywords: Immobilization; Lipase; Enantioselectivity; Epoxide; Menthyl butyrate
Depositing User: Najwani Amir Sariffudin
Date Deposited: 12 May 2010 16:54
Last Modified: 28 Sep 2016 12:05
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.foodchem.2007.04.026
URI: http://psasir.upm.edu.my/id/eprint/6144
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