Citation
Yaakub, Zulkifli
(2006)
Genotypic Characterization of Vibrio Sp. Isolated from Cockles Obtained in Padang, Indonesia.
Masters thesis, Universiti Putra Malaysia.
Abstract
Vibrio parahaemoi'yticus is a gram negative bacterium that naturally found in warm
marine environments. They commonly live in shellfish, oysters and cockles and cause
gastrointestinal illness in humans. Most people become infected by eating raw or
undercooked shellfish. In this study, 32 isolates of V: parahaemolyticus were isolated
Erom cockles obtained from Padang, Indonesia. Presumptive identification of the isolates
was performed by the following analysis: antibiotic resistance, plasmid profiling,
specific PCR, random amplified polymorphic DNA (RAPD)-PCR and enternbacterial
repetitive intergenic consensus (ERIC)-PCR. In the antibiotic resistance test, 16 types of
antibiotics were tested against this bacterium. The isolates of V: parahemolyticus
showed variable antibiotic resistance pattern. They are most commonly resistant to
amoxycillin, bacitracin, penicillin, teicoplanin, ampicillin and carbenicillin. In the
plasmid profiling analysis, 14 different profiles were determined. The most common
profiles for the isolates is profile 110.2 which contains 4 plasmids with 5.6,7.2,42.4 and
54.0 kilobase pairs respectively. For toxR detection, all of the isolates were positive but
they gave negative results in both tdh and trh detection. This indicated that they have
less potential to cause diseases. They also showed high diversity in RAPD-PCR and also
ERIC-PCR analysis. They showed many differences in the polymorphism pattern and
can be divided into 5 major clusters in RAPD-PCR and 4 major clusters in ERIC-PCR.
Application of phenotype and genotype based methods provides information about
distribution, epidemiology and pathogenesis of V. parasympathetics. The data is
important to identify sources of infection of this bacterium in the case of outbreaks
occurrence in humans. This study also gives us preliminary information in monitoring
the potential infection risk. It also can increase our awareness about the infection
potential of these bacteria.
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