Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia

Mohamed Zulperi, Zarirah and Omar, Abdul Rahman and Arshad, Siti Suri (2009) Sequence and phylogenetic analysis of S1, S2, M, and N genes of infectious bronchitis virus isolates from Malaysia. Virus Genes, 38 (3). pp. 383-391. ISSN 0920-8569

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Official URL: http://dx.doi.org/10.1007/s11262-009-0337-2

Abstract

Two Malaysian infectious bronchitis virus isolates, MH5365/95 and V9/04 were characterized based on sequence and phylogenetic analyses of S1, S2, M, and N genes. Nucleotide sequence alignments revealed many point mutations, short deletions, and insertions in S1 region of both IBV isolates. Phylogenetic analysis of S1 gene and sequences analysis of M gene indicated that MH5365/95 and V9/04 belong to non-Massachusetts strain. However, both isolates share only 77% identity. Analysis based on S1 gene showed that MH5365/95 shared more than 87% identity to several Chinese strains. Meanwhile, V9/04 showed only 67–77% identity to all the previously studied IBV strains included in this study suggesting it is a variant of IBV isolate that is unique to Malaysia. Phylogenetic analysis suggests, although both isolates were isolated 10 years apart from different states in Malaysia, they shared a common origin. Analysis based on S2 and N genes indicated that both strains are highly related to each other, and there are fewer mutations which occurred in the respective genes.

Item Type:Article
Keyword:Infectious bronchitis virus, S1, S2, M and N gene sequences, Phylogenetics analysis
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:Springer Science+Business Media, LLC 2009
DOI Number:10.1007/s11262-009-0337-2
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11262-009-0337-2
ID Code:5985
Deposited By: Azizan Arshad
Deposited On:06 May 2010 08:34
Last Modified:19 Aug 2010 04:44

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