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Plant regeneration of Brassica oleracea subsp. italica (Broccoli) CV Green Marvel as affected by plant growth regulators

Ravanfar, Seyed Ali, Abdul Aziz, Maheran, Abdul Kadir, Mihdzar, Abdul Rashid, Azmi and Torabi Sirchi, Mohammad Hossein (2009) Plant regeneration of Brassica oleracea subsp. italica (Broccoli) CV Green Marvel as affected by plant growth regulators. African Journal of Biotechnology, 8 (11). 2523-2528 . ISSN 1684–5315

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Abstract

Hypocotyls and shoot tips were used as explants in in vitro plant regeneration of broccoli (Brassica oleracea subsp.italica) cv. Green Marvel. The explants were excised from sterile germinated seedlings and placed on shoot induction medium containing basal salts of Murashige and Skoog (MS) and various concentrations of 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA). The highest percentage of hypocotyl explant producing shoot (96.67%) and the highest mean number of shoots produced per hypocotyl explant (6.03) were obtained on 3 mgL-1 BAP. Meanwhile, the highest percentage of shoot tip explant producing shoot (100%) and highest number of shoot produced per shoot tip explant (3.76) were recorded on 5 mgL-1 BAP. For rooting of shoots, NAA, indoleacetic acid (IAA) and indolebutyric acid (IBA) at 0, 0.2 and 1 mgL-1 were applied. Highest percentage of shoots with roots (100%) and highest mean number of roots produced per shoot (6.5) occurred on medium with 0.2 mgL-1 IBA, while the maximum root length (2.46 cm) was attained on MS medium without plant growth regulator (MSO). Plantlets were successfully acclimatized in potting medium containing peatmoss, perlite, and vermiculite (3:1:1).

Item Type:Article
Keyword:α-naphthaleneacetic acid, 6-benzylaminopurine, Broccoli, in vitro culture, Indole-3-butyric acid, Multiple shoot formation
Faculty or Institute:Faculty of Agriculture
Publisher:Academic Journals
ID Code:5591
Deposited By: Norhazura Hamzah
Deposited On:14 Apr 2010 06:24
Last Modified:27 May 2013 07:23

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