Citation
Abstract
The gelatin sources have become a controversial issue with regard to religious and health concern. Thus, the aims of this study were to develop and evaluate the efficiency of polyclonal antibodies against peptide immunogen of collagen α2 (I) chain for determination of gelatin sources in confectionery products by competitive indirect enzyme-linked immunosorbent assay (ELISA). Collagen α2 (I) chain protein showed resistance against heat treatment and detectable in certain commercial products when analysed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE). The established ELISA exhibited low cross-reactivity to fish and chicken gelatin. The IC50 value was 0.39 μg mL−1, and the limit of detection (IC10) was 0.05 μg mL−1. There were no false-positive results from forty-eight commercially processed products. The present method is useful for determination of gelatin in confectionery products.
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Official URL or Download Paper: http://onlinelibrary.wiley.com/wol1/doi/10.1111/ij...
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Medicine and Health Science Halal Products Research Institute |
| DOI Number: | https://doi.org/10.1111/ijfs.12971 |
| Publisher: | Institute of Food Science and Technology |
| Keywords: | Collagen α2 (I) chain; Enzyme-linked immunosorbent assay; Gelatin; Polyclonal antibody; SDS-PAGE |
| Depositing User: | Mohd Hafiz Che Mahasan |
| Date Deposited: | 14 Sep 2017 03:04 |
| Last Modified: | 15 Mar 2018 07:31 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/ijfs.12971 |
| URI: | http://psasir.upm.edu.my/id/eprint/55492 |
| Statistic Details: | View Download Statistic |
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