Secretary IgA Concentrations and plasma Cell Count Changes Associated with the Estrous Cycle in Ewes

Morteta H., Al-Medhtiy and Abu Bakar Zakaria, Md Zuki and Mohamed Mustapha, Noordin and Agungpriyono, Srihadi (2009) Secretary IgA Concentrations and plasma Cell Count Changes Associated with the Estrous Cycle in Ewes. American Journal of Animal and Veterinary Sciences, 4 (1). pp. 21-25. ISSN 1557-4555

Full text not available from this repository.

Official URL: http://dx.doi.org/10.3844/AJAV.2009.21.25

Abstract

Problems statement: The level of uterine Secretory-IgA (S-IgA) and numbers of plasma cells was measured to observe the differences between two stages of estrous cycle (follicular and luteal phase) in the healthy cycling non pregnant ewes. Approach: Twelve ewes were used in this study and they were divided into two groups of 6 animals each according to the stages of estrous cycle. All ewes were subjected to estrous synchronization and allowed to undergo one natural estrous cycle after the removal of the sponge. All animals were then slaughtered at the end of the experiment. The uterine mucus was collected by flushing with a mixture of protease inhibitor cocktail in distilled water. For both stages, the level of uterine S-IgA was quantified by using ELISA and Methyl Green Pyronine staining was used to observe the plasma cell in the tissues of the uterine horn and oviduct of ewe’s genital tract. Results: The results were analyzed by independent sample t-test and presented as mean±SEM. This study showed the relationship of the estrous cycle stages to uterine S-IgA concentration (μg mL-1) and populations of plasma cell in the healthy non-pregnant cycling ewes. The concentration (μg mL-1) of S-IgA (0.20±0.01) in the follicular phase was highly significant (p<0.01) as compared with the luteal phase (0.17±0.002). In addition, the populations of the plasma cells were significantly higher (p<0.01) in the uterine horn (4.97±0.32) and oviduct (3.82±0.33) during follicular phase compared to the luteal phase (3.87 ±0.30) and (1.90±0.21), respectively. Conclusion: The main reason for the immunosuppression during the luteal phase did not fully justified, especially with the presence of potential acquired infection during coitus in the follicular phase and at the same time immune system should decrease accordingly to prevent newly attached fetus rejection by the mother immune system.

Item Type:Article
Keyword:S-IgA, plasma cells, estrous cycle, uterine lavage, ewes
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:Science Publications
DOI Number:10.3844/AJAV.2009.21.25
ID Code:5480
Deposited By: Azizan Arshad
Deposited On:12 Apr 2010 02:35
Last Modified:19 Aug 2010 04:58

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 12 Apr 2010 02:35.

View statistics for "Secretary IgA Concentrations and plasma Cell Count Changes Associated with the Estrous Cycle in Ewes"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.