Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter

Mat Yunus, Abdul Masani and Ghulam Kadir, Ahmad Parveez and Abang Masli, Dayang Izawati and Chan, Pek Lan and Abdullah, Siti Nor Akmar (2009) Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter. Plasmid, 62 (3). pp. 191-200. ISSN 0147-619X

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1016/j.plasmid.2009.08.002

Abstract

One of the targets in oil palm genetic engineering programme is the production of polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-valerate (PHBV) in the oil palm leaf tissues. Production of PHB requires the use of phbA (β-ketothiolase type A), phbB (acetoacetyl-CoA reductase) and phbC (PHB synthase) genes of Ralstonia eutropha, whereas bktB (β-ketothiolase type B), phbB, phbC genes of R. eutropha and tdcB (threonine dehydratase) gene of Escherichia coli were used for PHBV production. Each of these genes was fused with a transit peptide (Tp) of oil palm acyl-carrier-protein (ACP) gene, driven by an oil palm leaf-specific promoter (LSP1) to genetically engineer the PHB/PHBV pathway to the plastids of the leaf tissues. In total, four transformation vectors, designated pLSP15 (PHB) and pLSP20 (PHBV), and pLSP13 (PHB) and pLSP23 (PHBV), were constructed for transformation in Arabidopsis thaliana and oil palm, respectively. The phosphinothricin acetyltransferase gene (bar) driven by CaMV35S promoter in pLSP15 and pLSP20, and ubiquitin promoter in pLSP13 and pLSP23 were used as the plant selectable markers. Matrix attachment region of tobacco (RB7MAR) was also included in the vectors to stabilize the transgene expression and to minimize silencing due to positional effect. Restriction digestion, PCR amplification and/or sequencing were carried out to ensure sequence integrity and orientation.

Item Type:Article
Keyword:Multiple-gene vectors, Oil palm leaf-specific promoter, Plastids, Polyhydroxybutyrate (PHB), Polyhyroxybutyrate-co-valerate (PHBV), Single-gene vectors
Faculty or Institute:Faculty of Agriculture
DOI Number:10.1016/j.plasmid.2009.08.002
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.plasmid.2009.08.002
ID Code:5380
Deposited By: Norhazura Hamzah
Deposited On:09 Apr 2010 01:28
Last Modified:28 Jul 2010 00:38

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 09 Apr 2010 01:28.

View statistics for "Construction of PHB and PHBV multiple-gene vectors driven by an oil palm leaf-specific promoter"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.