UPM Institutional Repository

Purification and characterization of nitric oxide inhibitory peptides from Actinopyga lecanora through enzymatic hydrolysis


Citation

Ghanbari, Raheleh and Ebrahimpour, Afshin and Zarei, Mohammad and Ismail, Amin and Abdul Hamid, Azizah and Saari, Nazamid (2016) Purification and characterization of nitric oxide inhibitory peptides from Actinopyga lecanora through enzymatic hydrolysis. Food Biotechnology, 30 (4). pp. 263-277. ISSN 0890-5436; ESSN: 1532-4249

Abstract

Actinopyga lecanora, commonly known as sea cucumber, is a rich protein source. This marine protein source was hydrolyzed using six proteases to generate anti-inflammatory hydrolysates and bioactive peptides. Bromelain hydrolysates after 1 h hydrolysis exhibited the highest nitric oxide (NO) inhibitory activity reflecting anti-inflammatory activity. A sequence of two fractionation methods was employed to fractionate the peptides based on their hydrophobicity using a semi-preparative RP-HPLC and isoelectric points using isoelectric focusing technique. Based on these fractionation methods, basic peptides with relatively higher hydrophobicity provided higher NO-inhibitory activity than did acidic peptides. Furthermore, using Q-TOF mass spectrometry; 12 peptide sequences were successfully identified. The inhibitory effect of the purified peptides from A. lecanora on NO production by lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was investigated. The three identified bioactive peptides, namely LREMLSTMCTARGA, AVGPAGPRG and VAPAWGPWPKG, exhibited the highest NO-inhibitory activity with values of 76.3, 66.6 and 69.9%, respectively. These results revealed that A. lecanora could be used as an economical protein source for the production of high-value bioactive peptides with potent anti-inflammatory activity using RAW 264.7 cell lines as model. These peptides may be useful ingredients in food and pharmaceutical applications.


Download File

[img]
Preview
PDF
Purification and characterization of nitric oxide inhibitory peptides from Actinopyga lecanora through enzymatic hydrolysis.pdf

Download (8kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1080/08905436.2016.1234391
Publisher: Taylor & Francis
Keywords: Actinopyga lecanora; Bioactive peptides; Enzymatic hydrolysis; NO-inhibitory activity; Anti-inflammation
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 09 Jan 2018 08:24
Last Modified: 09 Jan 2018 08:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/08905436.2016.1234391
URI: http://psasir.upm.edu.my/id/eprint/53702
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item