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RAPD analysis of colchicine induced variation of the Dendrobium Serdang beauty

Khosravi, Alireza and Abdul Kadir, Mihdzar and Kadzemin, S. B. and Qamaruz Zaman, Faridah and De Silva, Angela Ee (2009) RAPD analysis of colchicine induced variation of the Dendrobium Serdang beauty. African Journal of Biotechnology, 8 (8). art. no. 1AEFFEB9384. pp. 1455-1465. ISSN 1684–5315

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Abstract

Variation was detected in Dendrobium Serdang Beauty V (DSB V) plantlets regenerated from protocorm like bodies (plbs) induced by various concentration levels of colchicines in the Murashige and Skoog media (MS) supplemented with 1.5 mg/L IBA. RAPD analysis detected 6 - 26% variation in the regenerants from the mother plant. The highest variation was obtained in regenerates treated with 25 mg/L colchicine, which also exhibited reduced regeneration rates from plbs and mean plantlet fresh weight. RAPD analysis also showed high polymorphism between the mutated regenerant DSB V, and 13 species of the Dendrobiumgenera, and 13 orchids across generas. However, despite the 26% colchicine induced variation in the regenerants, all RAPD analysis revealed that DSB V was closely related to the mother plant. Thus, the RAPD technique is favourable for variation detection as it was sensitive enough to detect variations at species level and among somaclonal variants in this study.

Item Type:Article
Keyword:Colchicine; Orchid; RAPD; Dendrobium
Faculty or Institute:Faculty of Agriculture
Faculty of Science
Publisher:Academic Journals
ID Code:5194
Deposited By: Norhazura Hamzah
Deposited On:07 Apr 2010 10:09
Last Modified:15 Nov 2017 14:17

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