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Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of Chaetomium globosum


Citation

Md. Shah, Umi Kalsom and Ariff, Arbakariya and Abdul Karim, Mohamed Ismail (1998) Saccharification of pretreated oil palm empty fruit bunch fiber using cellulase of Chaetomium globosum. Journal of Agricultural and Food Chemistry, 46 (8). pp. 3359-3364. ISSN 0021-8561; ESSN: 1520-5118

Abstract

The effectiveness of different chemical and physical pretreatments to alter cellulose structure and to reduce hemicellullose and lignin contents in oil palm empty fruit bunch (OPEFB) fibers for subsequent use as substrate for enzymatic saccharification was studied. The saccharification was carried out using concentrated cellulase preparation from culture broth of Chaetomium globosum strain 414 containing 10 U/mL FPase, 285 U/mL CMCase, and 60 U/mL β-glucosidase. The use of 0.5% NaOH to treat OPEFB by soaking at 30 °C for 4 h gave the highest rate and degree of hydrolysis followed by 0.5% HNO3, HCl, EDA, and EDTA. Autoclaving the chemically treated OPEFB fiber at 121 °C for 5 min improved the hydrolysis by 2-fold. The improvement in hydrolysis was related to a decrease in the hemicellulose and lignin contents and an increase in the cellulose content. The qualitative hydrolysis yield for autoclaved OPEFB fiber treated with 2% NaOH was 85.9%. During saccharification of OPEFB using cellulase of C. globosum, the amount of glucose produced was higher while the amount of cellobiose produced was lower than those obtained with commercial cellulase of Trichoderma viride.


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Official URL or Download Paper: http://pubs.acs.org/doi/abs/10.1021/jf971098c

Additional Metadata

Item Type: Article
Divisions: Universiti Putra Malaysia
DOI Number: https://doi.org/10.1021/jf971098c
Publisher: American Chemical Society
Keywords: Cellulase; Endoglucanase; Cellobiohydrolase; β-glucosidase; Chaetomium globosum; Cellulosic material; Oil palm empty fruit bunch
Depositing User: Nabilah Mustapa
Date Deposited: 05 Sep 2017 08:38
Last Modified: 05 Sep 2017 08:38
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1021/jf971098c
URI: http://psasir.upm.edu.my/id/eprint/51670
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