Keyword Search:


Bookmark and Share

Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves

Abdul Razak, Mohd Farhan (2012) Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves. Masters thesis, Universiti Putra Malaysia.

[img] PDF
720Kb

Abstract

Orthosiphon stamineus, also known as Misai Kucing, is one of the invaluable medicinal plants originated from Southeast Asia. O. stamineus leaves were used in plenty of applications related to the medicinal purposes and are believed to cure certain disease such as hypertension, gout and fever. However, very little research done with regards to ascertain optimum extraction process and quality extract of O. stamineus leaves. This research studied a solid- liquid extraction (SLE) process which are involved in processing of bioactive extract from O. stamineus leaves. Different experiments were carried out to determine the effects of various operating parameters on the qualitative and quantitative aspects of O. stamineus leaves. Rosmarinic acid (RA) and Sinensetin (SEN) were selected as the quality indicators of the product because these two compounds were played a major role in the bioactivities including anti- inflammatory, anti- oxidant and anti- pyretic of O. stamineus leaves. Water is most suitable solvent for extracting O. stamineus leaves compared to ethanol, ethyl acetate, and hexane. This was because water gave the highest extraction yield as well as quality extract that related to RA and SEN. Besides, water also indicated highest antioxidant and anti- inflammatory compared to other solvents. The optimum extraction condition of O. stamineus leaves was determined as 60°C and the ratio of water to solid is 10:1 (ml:g) to avoid degradation of SEN based on analysis using Response Surface Methodology (RSM) technique. of 6 hours were found to be optimum based on analysis of Response Surface Methodology (RSM). Two hours of extraction duration was selected as optimum extraction time based on kinetic study. The activation energy of the extraction is 36.57 kJ mol-1.

Item Type:Thesis (Masters)
Subject:Biochemical engineering
Subject:Solid-liquid interfaces
Chairman Supervisor:Professor Luqman Chuah Abdullah, PhD
Call Number:FK 2012 113
Faculty or Institute:Faculty of Engineering
ID Code:48466
Deposited By: Haridan Mohd Jais
Deposited On:09 Sep 2016 10:19
Last Modified:09 Sep 2016 10:19

Repository Staff Only: Edit item detail

Document Download Statistics

This item has been downloaded for since 09 Sep 2016 10:19.

View statistics for "Optimisation of solid-liquid extraction of bioactive compounds from Orthosiphon stamineus benth leaves"