Simple Search:

Mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake


Citation

Buchi, Iluyemi Florence and Musa, Mohamed Hanafi (2009) Mycelial growth interactions and mannan-degrading enzyme activities from fungal mixed cultures grown on palm kernel cake. African Journal of Biotechnology, 8 (10). art. no. 7B46A948018. pp. 2283-2288. ISSN 1684–5315

Abstract / Synopsis

Palm kernel cake (PKC), a by-product of the palm kernel oil extraction process contains mannan as its main polysaccharide. Mixed culture microbial degradation may enhance mannan-degrading enzymes production. Therefore, the objective of the study was to examine the nature of mycelial interactions and corresponding production of mannan-degrading enzymes of PKC. Fungal interactions was carried out using Sclerotium rolfsii and Aspergillus niger co-cultured with three Trichoderma strains (Trichoderma harzianum, Trichoderma longiobrachiatum and Trichoderma koningii) on potato dextrose agar (PDA) in disposable petri-dishes. Measurements of growth diameters were taken on days 2 and 13. For mannan-degrading enzyme production, single and co-cultures of these fungi were carried out under submerged cultivation for 13 days with PKC as the carbon source. About 57% of observed interactions on PDA were deadlock, 29% replacement and 14% intermingling. In Trichoderma sp./A. niger mixed cultures, there was an overall significant enhancement of enzyme: 2 to 200 fold (β-D-mannanase), 8 to 25 fold (β-mannosidase) and from no change to 15 fold increase (α-galactosidase). There was no obvious relationship between enzyme production and protein yield. However, co-culturing of A. niger with the Trichoderma strains showed an enhancement of mannan-degrading enzyme activities without reducing biomass yield.


Download File

[img] PDF
Mycelial_growth_interactions.pdf
Restricted to Repository staff only

Download (143kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Institute of Tropical Agriculture
Publisher: Academic Journals
Keywords: α-galactosidase; β-D-mannanase; β-mannosidase; Co-cultures; Fungi; Palm kernel cake
Depositing User: Norhazura Hamzah
Date Deposited: 31 Mar 2010 14:24
Last Modified: 09 Nov 2017 16:17
URI: http://psasir.upm.edu.my/id/eprint/4790
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item