Molecular characterization of Pseudomonas aeruginosa UPM P3 from oil palm rhizosphere

Meon, Sariah and Azadeh, B. F. (2009) Molecular characterization of Pseudomonas aeruginosa UPM P3 from oil palm rhizosphere. American Journal of Applied Sciences , 6 (11). pp. 1915-1919. ISSN 1546-9239

Full text not available from this repository.

Official URL: http://dx.doi.org/10.3844/ajassp.2009.1915.1919

Abstract

Problem statement: Pseudomonas aeruginosa has been used in agriculture as biological agents. It has shown substantial control of a variety of soil-borne plant pathogens including Macrophomina phaseolina, Botrytis cinerea, Rhizoctonia solani, Colletotrichum truncatum, Pythium, Fusarium and others. Species aggregate of Pseudomonas aeruginosa strain UPM P3 was shown to have potential as a biocontrol agent against Ganoderma boninense, the causal agent of Basal Stem Rot (BSR) of oil palm. However, P. aeruginosa is also an opportunistic pathogen. It typically infects the pulmonary tract, urinary tract, burns, wounds and also causes other blood infections. The objective of this study was to carry out DNA fingerprinting for strain differentiation to differentiate between pathogenic and non-pathogenic forms of P. aeruginosa strain UPM P3. Approach: Genotype characterization was carried out by amplification of the recA gene using specific primers, purified using QIA Quick PCR purification Kit and sent for sequencing. Multiple sequence alignments were performed on the selected closely related sequence accessions using CLUSTAL W software. The recA gene was used for phylogenetic and PCR-RFLP studies. Results: From the phylogenetic tree, UPM P3 has more than 90% similarity with Pseudomonas aeruginosa strains: PAM7, PAO1, UCBPP-PA14 and PA7. UPM P3 was further digested with restriction enzymes; PvuII, BsrI, ZraI, FokI and SgrAI. RFLP results showed that strain UPM P3 has close similarity with strain PAO1 of Pseudomonas aeruginosa. Conclusion: Strain PAO1 is commonly associated with strains of medical, human or plant pathogens and agricultural environment. Common habitats include soil, hosts, aquatic environment and wastewater and also a common contaminant of public places. Thus the use of Pseudomonas aeruginosa strain UPMP3 as a biological control candidate in agriculture has to be monitored.

Item Type:Article
Keyword:Basal Stem Rot (BSR), Ganoderma boninense, PAO1, Pseudomonas aeroginusa, recA gene, RFLP, UPM P3
Faculty or Institute:Faculty of Agriculture
DOI Number:10.3844/ajassp.2009.1915.1919
ID Code:4712
Deposited By: Norhazura Hamzah
Deposited On:30 Mar 2010 04:14
Last Modified:30 Mar 2010 04:15

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 30 Mar 2010 04:14.

View statistics for "Molecular characterization of Pseudomonas aeruginosa UPM P3 from oil palm rhizosphere"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.