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Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach


Citation

Namvar, Farideh and Rahman, Heshu Sulaiman and Mohamad, Rosfarizan and Rasedee, Abdullah and Swee, Keong Yeap and Chartrand, Max Stanley and Azizi, Susan and Mohd Tahir, Paridah (2015) Apoptosis induction in human leukemia cell lines by gold nanoparticles synthesized using the green biosynthetic approach. Journal of Nanomaterials, 2015. art. no. 642621. pp. 1-10. ISSN 1687-4110; ESSN: 1687-4129

Abstract

Gold nanoparticles were grown on Sargassum muticum water extract (S-GNPs) using the green biosynthetic approach. The nanoparticles were characterized using UV-visible spectroscopy, zeta potential, and transmission electron microscopy (TEM). The resulting S-GNPs were spherical and crystalline with a size of <10 nm. The in vitro anticancer activity was demonstrated in human leukemia cell lines. The cancer cells were treated with different concentrations of S-GNPs, and calorimetric (MTT) assay used for the cytotoxicity test, which resulted in an IC50 value of 4.22 ± 1.12, 5.71 ± 1.4, 6.55 ± 0.9, and 7.29 ± 1.7 μg/mL for each of the K562, HL-60, Jurkat, and CEM-ss cells, respectively. Thus, the K562 was selected for the next experiments. Furthermore, apoptosis induction was confirmed by Hoechst 33342, annexin V staining, and caspase-3/-9 activity tests. The cell cycle analysis exhibited a significant increase in the accumulation of S-GNPs treated cells at the sub-G1 phase, demonstrating the induction of apoptosis by S-GNPs. The nature of the inhibition of cancer cell growth by S-GNPs could open the way for further research in the design of green synthesis therapeutic agents, particularly in nanomedicine, for the treatment of cancer.


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Official URL or Download Paper: http://www.hindawi.com/journals/jnm/2015/642621/

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Faculty of Veterinary Medicine
Institute of Bioscience
Institute of Tropical Forestry and Forest Products
DOI Number: https://doi.org/10.1155/2015/642621
Publisher: Hindawi Publishing Corporation
Keywords: Nanoparticles; Biosynthetic
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 21 Sep 2016 04:31
Last Modified: 21 Sep 2016 04:31
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2015/642621
URI: http://psasir.upm.edu.my/id/eprint/43810
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