Keyword Search:


Bookmark and Share

A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes

Azmi, Nur Ellina and Ramli, Noor Izaanin and Abdullah, Jaafar and Abdul Hamid, Mohammad Azmi and Sidek, Hamidah and Abd Rahman, Samsulida and Ariffin, Nurhayati and Yusof, Nor Azah (2015) A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes. Biosensors and Bioelectronics, 61 (2015). pp. 129-133. ISSN 0956-5663

[img] PDF (Abstract)
99Kb

Official URL: http://www.sciencedirect.com/science/article/pii/S...

Abstract

A novel optical detection system consisting of combination of uricase/HRP–CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125–1000 µM with detection limit of 125 µM.

Item Type:Article
Keyword:Quantum dots; Enzyme; Uric acid; Fluorescence; Quenching
Faculty or Institute:Faculty of Science
Institute of Advanced Technology
Publisher:Elsevier
DOI Number:10.1016/j.bios.2014.07.056
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.bios.2014.07.056
ID Code:43602
Deposited By: Mohd Hafiz Che Mahasan
Deposited On:21 Jul 2016 17:30
Last Modified:21 Jul 2016 17:30

Repository Staff Only: Edit item detail