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A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes


Citation

Azmi, Nur Ellina and Ramli, Noor Izaanin and Abdullah, Jaafar and Abdul Hamid, Mohammad Azmi and Sidek, Hamidah and Abd Rahman, Samsulida and Ariffin, Nurhayati and Yusof, Nor Azah (2015) A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes. Biosensors and Bioelectronics, 61. pp. 129-133. ISSN 0956-5663; ESSN: 1873-4235

Abstract / Synopsis

A novel optical detection system consisting of combination of uricase/HRP–CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125–1000 µM with detection limit of 125 µM.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Institute of Advanced Technology
DOI Number: 10.1016/j.bios.2014.07.056
Publisher: Elsevier
Keywords: Quantum dots; Enzyme; Uric acid; Fluorescence; Quenching
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 21 Jul 2016 17:30
Last Modified: 01 Nov 2017 17:18
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.bios.2014.07.056
URI: http://psasir.upm.edu.my/id/eprint/43602
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