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A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection

Abd Rashid, Jahwarhar Izuan and Yusof, Nor Azah and Abdullah, Jaafar and Hashim, Uda and Hajian, Reza (2015) A novel disposable biosensor based on SiNWs/AuNPs modified-screen printed electrode for dengue virus DNA oligomer detection. IEEE Sensors Journal, 15 (8). pp. 4420-4427. ISSN 1530-437X

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Abstract

In this study, a disposable screen-printed gold electrode (SPGE) utilized of silicon nanowires (SiNWs) and gold nanoparticles as sensing material was fabricated for detection of DNA oligomers related to dengue virus. Firstly, SiNWs/AuNPs-SPGE was developed by the dispersion of SiNWs in 3-Aminopropyltriethoxysilane (APTES, 0.5 %) onto bare SPGE. Secondly, the AuNPs decoration on SiNWs-SPGE surface was functionalized using dithiopropionic acid (DTPA) through a self-assembly monolayer (SAM) technique. The electrochemical response of methylene blue (MB) as a redox indicator towards synthetic DNA oligomer after hybridization on SiNWs/AuNPs-SPGE was recorded by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The results demonstrated that the reduction peak current of MB was significantly decreased after DNA hybridization process. In addition, the developed biosensor showed a good storage stability and could achieve a linear range of 1.0 × 10-11-1.0 × 10-7 M (R= 0.98) with the detection limit of 1.63× 10-12 M.

Item Type:Article
Keyword:Silicon nanowires; Gold nanoparticles; DNA hybridization; Dengue virus
Faculty or Institute:Faculty of Science
Publisher:IEEE
DOI Number:10.1109/JSEN.2015.2417911
Altmetrics:httphttp://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1109/JSEN.2015.2417911
ID Code:43536
Deposited By: Mohd Hafiz Che Mahasan
Deposited On:29 Jun 2016 11:15
Last Modified:29 Jun 2016 11:15

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