The best method for isolated total RNA from durian tissues

Citation

Ky, Huynh and Yeap, Swee Keong and Napis, Suhaimi (2012) The best method for isolated total RNA from durian tissues. International Food Research Journal, 19 (3). pp. 1181-1183. ISSN 1985-4668; ESSN: 2231-7546

Abstract

Plant tissues, especially durian tissues contain high content of polysaccharides, polyphenols and other secondary metabolites which can co-precipitate with RNA causing problem in further transcriptomic study. In this experiment, three basic chaotic agents, CTAB, SDS and guanidine are used in three basic protocols for RNA isolation. The effectiveness of each method was determined by spectrophotometer, denaturing agarose gels analysis and northern blot hybridization. CTAB combining with additional sodium acetate precipitation step showed highest yield and best quality of isolated RNA which was free from contaminations of polysaccharides, polyphenols and other secondary metabolites. Furthermore, the total RNA from 4-month old durian flesh of clone D24 was successfully used to construct a cDNA library. In conclusion, CTAB method is effective to isolate total RNA on various types of durian tissues for further gene expression analysis.

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Official URL or Download Paper: http://www.ifrj.upm.edu.my/19%20%2803%29%202012/%2...

Additional Metadata

Item Type:	Article
Divisions:	Faculty of Biotechnology and Biomolecular Sciences
Publisher:	Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords:	Ctab; Durio zibethinus murr.; Guanidine; Sds; Total RNA
Depositing User:	Najah Mohd Ali
Date Deposited:	30 Nov 2015 04:34
Last Modified:	30 Nov 2015 04:34
URI:	http://psasir.upm.edu.my/id/eprint/41007
Statistic Details:	View Download Statistic

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