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Immunomodulatory effects of Potentilla indica and Dendrophthoe pentandra on mice splenocytes and thymocytes

Ang, Hui Ying and Subramani, Tamilselvan and Yeap, Swee Keong and Omar, Abdul Rahman and Ho, Wan Yang and Abdullah, Mohd Puad and Mohamed Alitheen, Noorjahan Banu (2014) Immunomodulatory effects of Potentilla indica and Dendrophthoe pentandra on mice splenocytes and thymocytes. Experimental and Therapeutic Medicine, 7 (6). pp. 1733-1737. ISSN 1792-0981; ESSN: 1792-1015

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Abstract

Immunomodulators are agents that are able to stimulate or inhibit the immune response. The leaf extracts from Potentilla indica and Dendrophthoe pentandra were analyzed in vitro for immunomodulatory activity and an MTT colorimetric assay was conducted to determine the proliferation of mice splenocytes and thymocytes. A bromodeoxyuridine assay was performed to analyze DNA synthesis and the Trypan blue exclusion method was conducted to evaluate the changes in total cell population. The results indicated that treatment with P. indica and D. pentandra produced a time‑ and dose-dependent increase in cell viability and proliferation. Following 72 h of treatment with P. indica and D. pentandra, thymocyte proliferation was augmented by 18 and 41%, respectively and splenocyte proliferation increased by 35 and 42%, respectively, when compared with untreated cells. The present study demonstrated that these extracts may act as potential immunostimulants and, thus, represent an alternative source of immunomodulatory compounds for the treatment of human immune‑mediated diseases.

Item Type:Article
Keyword:Bromodeoxyuridine assay; Immunomodulatory; Mistletoe; MTT; Rosaceae; Spleen; Thymus
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Publisher:Spandidos Publications
DOI Number:10.3892/etm.2014.1657
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3892/etm.2014.1657
ID Code:37937
Deposited By: Nabilah Mustapa
Deposited On:29 Dec 2015 20:25
Last Modified:29 Dec 2015 20:25

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