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Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract

Albaayit, Shaymaa Fadhel Abbas and Abba, Yusuf and Abdullah, Rasedee and Abdullah, Noorlidah (2014) Evaluation of antioxidant activity and acute toxicity of Clausena excavata leaves extract. Evidence-Based Complementary and Alternative Medicine, 2014 . art. no. 975450. pp. 1-10. ISSN 1741-427X; ESSN: 1741-4288

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Clausena excavata (Lour.), locally known as “Kemantu hitam,” is a common plant in Malaysian folklore medicine. This study evaluated the antioxidant properties of the solvent extracts of C. excavata leaves and determined the acute toxicity of methanolic extract C. excavata (MECE) leaves in Sprague-Dawley rats. Harvested leaves were dried and subjected to solvent extraction using petroleum ether, chloroform, ethyl acetate and methanol in succession. The antioxidant activity of each extract was determined using the ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryl dihydrazyl (DPPH) radical scavenging activity. The total phenolic content (TPC) and total flavonoids content (TFC) were estimated by Folin-Ciocalteu and ethanolic aluminium chloride method, respectively. The chloroform extract was found to be highest in flavonoid content, while the methanolic extract showed the highest TPC and antioxidant activity. There was no mortality in rats treated with MECE leaves even at a high dose of 5000 mg/kg body weight. However, the MECE leaves produced mild to moderate pathological changes in the liver and kidneys, shown by mild degenerative changes and leucocyte infiltration. The extract did not affect the haematological parameters or relative weights of the liver or kidneys. Overall, the MECE leaves have potent antioxidant activity and are presumed safe to be used orally as health-promoting product at low to moderate doses.

Item Type:Article
Keyword:Kemantu hitam; Clausena excavata; Acute toxicity; Antioxidant activity
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:Hindawi Publishing Corporation
DOI Number:10.1155/2014/975450
ID Code:37520
Deposited By: Nabilah Mustapa
Deposited On:18 Dec 2015 10:52
Last Modified:18 Dec 2015 10:52

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