Keyword Search:


Bookmark and Share

Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: molecular detection and histopathological evaluation

Abdullah, Faez Firdaus Jesse and Adamu, Lawan and Abba, Yusuf and Tijjani, Abdulnasir and Mohammed, Konto and Omar, Abdul Rahman and Saharee, Abdul Aziz (2014) Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: molecular detection and histopathological evaluation. Research Opinions in Animal & Veterinary Sciences, 4 (10). pp. 535-539. ISSN 2221-1896; ESSN: 2223-0343

[img] PDF (Abstract)
83Kb

Official URL: http://www.roavs.com/archive/vol-4-issue-10-2014.h...

Abstract

Haemorrhagic septicaemia (HS) is an important disease of cattle and buffaloes worldwide, caused by Pasteurella multocida type B: 2 and type E. Here, we evaluated the clinical signs, gross and histopathological changes as well as molecular detection of P. multocida in various organs of mice following oral inoculation with graded doses of the bacterium. A total of 32 healthy mice were divided into four groups of 8 mice each where each group was inoculated orally with 0.4ml of P. multocida type B: 2 at the dose of 10³ (Group 1), 105 (Group 2) and 107 (Group 3) colony forming unit (CFU) respectively, while Group 4 served as a control. Post-mortem examination was conducted and gross lesions were observed. Tissue samples were collected for molecular detection and histo-pathological processing. All mice from the challenged groups showed almost no significant (P>0.05) clinical signs. Evidently, the severity of histo-pathological lesions in various organs was significantly high in groups 2 and 3. Through polymerase chain reaction detection (PCR), P. mulltocida type B: 2 was detected in all the experimental groups. Overall, the oral inoculation of mice at different doses of P. multocida type B: 2 results in clinical signs and histopathological lesions of haemorrhagic septicaemia infection.

Item Type:Article
Keyword:Haemorrhagic septicaemia; Pasteurella multocida type B; Mice; Histopathology; Lesions; Polymerase chain reaction (PCR); Dose dependent
Faculty or Institute:Faculty of Veterinary Medicine
Publisher:KPK Publishers
ID Code:37232
Deposited By: Nabilah Mustapa
Deposited On:24 Aug 2015 18:46
Last Modified:27 Aug 2015 15:31

Repository Staff Only: Edit item detail