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Detection and molecular characterization of infectious spleen and kidney necrosis virus from major ornamental fish breeding states in Peninsular Malaysia


Citation

Subramaniam, Kuttichantran and Mohamed Din, Mohamed Shariff and Omar, Abdul Rahman and Bejo, Mohd Hair and Ong, Bee Lee (2014) Detection and molecular characterization of infectious spleen and kidney necrosis virus from major ornamental fish breeding states in Peninsular Malaysia. Journal of Fish Diseases, 37 (7). pp. 609-618. ISSN 0140-7775; ESSN: 1365-2761

Abstract

‘Gold standard’ OIE reference PCR assay was utilized to detect the presence of infectious spleen and kidney necrosis virus (ISKNV) in freshwater ornamental fish from Malaysia. From total of 210 ornamental fish samples representing 14 species, ISKNV was detected in 36 samples representing 5 fish species. All positive cases did not show any clinical signs of ISKNV. Three restriction enzymes analyses showed that the fish were infected by identical strains of the same virus species within Megalocytivirus genus. Major capsid protein (MCP) genes of 10 ISKNV strains were sequenced and compared with 9 other reference nucleotide sequences acquired from GenBank. Sequence analysis of MCP gene showed that all strains detected in this study were closely related to the reference ISKNV with nucleotide sequence identity that was ranging from 99.8% to 100%. In addition, phylogenetic analysis of MCP gene revealed that viruses from genus Megalocytivirus can be divided into three genotypes: genotype 1 include reference ISKNV and all other strains that were detected in this study, genotype 2 include viruses closely related to red sea bream iridovirus (RSIV), and genotype 3 include viruses closely related turbot reddish body iridovirus (TRBIV).


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.1111/jfd.12152
Publisher: John Wiley & Sons
Keywords: Infectious spleen and kidney necrosis virus; Major capsid protein gene; Megalocytivirus; Ornamental fish
Depositing User: Nabilah Mustapa
Date Deposited: 02 Jun 2015 11:18
Last Modified: 26 Jan 2016 01:41
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/jfd.12152
URI: http://psasir.upm.edu.my/id/eprint/37008
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