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Canine-specific PCR assay targeting cytochrome b gene for the detection of dog meat adulteration in commercial frankfurters

Ali, Md. Eaqub and Rahman, Md. Mahfujur and Abd Hamid, Sharifah Bee and Mustafa, Shuhaimi and Bhassu, Subha and Hashim, Uda (2014) Canine-specific PCR assay targeting cytochrome b gene for the detection of dog meat adulteration in commercial frankfurters. Food Analytical Methods, 7 (1). pp. 234-241. ISSN 1936-9751; ESSN:1936-976X

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Abstract

This report described a cytochrome b (cytb)-based polymerase chain reaction (PCR) assay for the detection of canine tissues in commercial frankfurters. Discriminating detection of canine derivatives in processed food products has important application in halal authentication as well as in health, religions, and fare trades. The assay based on a pair of canine-specific primers that targeted a 100 bp region of canine mithochondrial-cytb gene which is present in multiple copies and highly conserved within the same species. The specificity of the assay was tested against dog and eight most common animal meat species as well as five plant species commonly found in frankfurter formulation. The stability and specificity of the assay were verified under different thermal processing conditions under pure and complex matrices. Three commercial brands of chicken and beef frankfurters were tested in triplicate, and specific PCR products were obtained only from deliberately contaminated formulations. The detection limit of the assay was 0.1 % (0.02 ng DNA) of canine meat spiked with other meats in a typical frankfurter formulation. Shorter amplicon length, superior stability, and higher sensitivity of the assay suggested its potential application in the screening of canine-origin biomaterials in processed food products.

Item Type:Article
Keyword:Canine-origin biomaterials; Commercial frankfurters; Halal authentication
Faculty or Institute:Halal Products Research Institute
Publisher:Springer
DOI Number:10.1007/s12161-013-9672-y
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s12161-013-9672-y
ID Code:36696
Deposited By: Nabilah Mustapa
Deposited On:25 May 2015 18:20
Last Modified:26 Aug 2015 15:23

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