Purification and Characterisation of ,B-l,3-giucanase from Trichodenna harzianum BIO 10671

Mustafa, Muskhazli and Ramli, Salfarina and Ithnin, Nalisha and Tohfah, Nor Farizan (2005) Purification and Characterisation of ,B-l,3-giucanase from Trichodenna harzianum BIO 10671. Pertanika Journal of Tropical Agricultural Science, 28 (1). pp. 23-31. ISSN 1511-3701

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Abstract

B-1,3-glucanase enzyme from culture filtrate of Trichoderma harzianum BIO 10671 was successively purified by precipitation with 80%acetonefollowed by anion-exchange chromatography on Neobar AQ and chromatofocusing on a Mono P HR 5/20 column. Two {3-1,3-glucanases of 32kDa and 66kDa were purified to homogeneity as judged by SDS-PAGE. The pH aptimum far the enzymes activity was pH 4-5 and maximum activity was obtained at 4YG. Enzyme activity was slightly inhibited by 20-45% in its activity by 20mM of Zn2+, Ca2+, Co2+, Mi+, Cu2+, Mn2+and Fe2+. The highest f3-1,3-glucanase hydrolysis activity was obtained on laminarin due to the similarity on f3-glucosidic bonds and followed on pustulan, glucan and cellulose, respectively.

Item Type:Article
Keyword:Tricoderma hanianum, ~1,3-g1ucanase, purification, characterisation, pH, metal ion
Publisher:Universiti Putra Malaysia Press
ID Code:3653
Deposited By: Farhana Yusof
Deposited On:01 Dec 2009 00:26
Last Modified:27 May 2013 07:10

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