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Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line


Citation

Rahman, Heshu Sulaiman and Abdullah, Rasedee and Abdul, Ahmad Bustamam and Allaudin, Zeenathul Nazariah and Othman, Hemn Hassan and Yeap, Swee Keong and How, Chee Wun and Wan Abd Ghani, Wan Nor Hafiza (2014) Zerumbone-loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukemia cell line. International Journal of Nanomedicine, 9 (1). pp. 527-538. ISSN 1176-9114; ESSN: 1178-2013

Abstract

This investigation evaluated the antileukemia properties of a zerumbone (ZER)-loaded nanostructured lipid carrier (NLC) prepared by hot high-pressure homogenization techniques in an acute human lymphoblastic leukemia (Jurkat) cell line in vitro. The apoptogenic effect of the ZER-NLC on Jurkat cells was determined by fluorescent and electron microscopy, Annexin V-fluorescein isothiocyanate, Tdt-mediated dUTP nick-end labeling assay, cell cycle analysis, and caspase activity. An MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) assay showed that ZER-NLC did not have adverse effects on normal human peripheral blood mononuclear cells. ZER-NLC arrested the Jurkat cells at G2/M phase with inactivation of cyclin B1 protein. The study also showed that the antiproliferative effect of ZER-NLC on Jurkat cells is through the intrinsic apoptotic pathway via activation of caspase-3 and caspase-9, release of cytochrome c from the mitochondria into the cytosol, and subsequent cleavage of poly (adenosine diphosphate-ribose) polymerase (PARP). These findings show that the ZER-NLC is a potentially useful treatment for acute lymphoblastic leukemia in humans.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.2147/IJN.S54346
Publisher: Dove Medical Press
Keywords: Zerumbone-loaded nanostructured lipid carrier; Cell cycle arrest; Apoptosis; Mitochondrial pathway
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 15 Feb 2016 03:36
Last Modified: 30 Oct 2019 07:13
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2147/IJN.S54346
URI: http://psasir.upm.edu.my/id/eprint/36045
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