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Isolation, identification and characterization of elevated phenol degrading Acinetobactersp. Strain AQ5NOL 1

Ahmad, Siti Aqlima and Syed, Mohd Arif and Mat Arif, Noorliza and Abd. Shukor, Mohd. Yunus and Shamaan, Nor Aripin (2011) Isolation, identification and characterization of elevated phenol degrading Acinetobactersp. Strain AQ5NOL 1. Australian Journal of Basic and Applied Sciences, 5 (8). pp. 1035-1045. ISSN 1991-8178

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Abstract

The increasing phenol and phenolic wastes necessitates the screening of bacteria that are able to degrade phenol. 115 bacterial isolates from several industrial sites and farms in Malaysia were screened for phenol degrading activity in minimal salt media (MSM) containing 0.5 gL-1 phenol. Thirty seven bacterial isolates exhibited phenol degrading activity and of this total, 6 isolates showed high phenol activity after 8 days of incubation. The isolate with the highest phenol degrading activity was subsequently identified as Acinetobacter sp. Strain AQ5NOL 1 based on BiologTM GN plates and partial 16S rDNA molecular phylogeny. The optimum conditions for achieving high phenol degradation were 0.04% (w/v) (NH4)2SO4, 0.01% (w/v) NaCl, pH 7, and temperature of 30°C. Acinetobacter sp. Strain AQ5NOL 1 was found to degrade phenol of up to 1500 mgL-1 concentrations under the optimized conditions. The isolation of Acinetobacter sp Strain AQ5NOL 1 provides an alternative for the bioremediation of phenol and phenolic wastes.

Item Type:Article
Keyword:Isolation; Characterization; Elevated phenol degrading activity; Acinetobacter sp.
Faculty or Institute:Faculty of Biotechnology and Biomolecular Sciences
Publisher:American-Eurasian Network for Scientific Information
ID Code:35283
Deposited By: Nurul Ainie Mokhtar
Deposited On:12 Oct 2016 12:21
Last Modified:12 Oct 2016 12:21

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