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Optimization of processing parameters for extraction of amylase enzyme from dragon (Hylocereus polyrhizus) peel using response surface methodology

Amid, Mehrnoush and Abd Manap, Mohd Yazid and Ahmad Zohdi, Nor Khanani (2014) Optimization of processing parameters for extraction of amylase enzyme from dragon (Hylocereus polyrhizus) peel using response surface methodology. The Scientific World Journal, 2014. art. no. 640949. pp. 1-12. ISSN 2356-6140; ESSN: 1537-744X

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Abstract

The main goal of this study was to investigate the effect of extraction conditions on the enzymatic properties of thermoacidic amylase enzyme derived from dragon peel. The studied extraction variables were the buffer-to-sample (B/S) ratio (1: 2 to 1: 6, w/w), temperature (-18°C to 25°), mixing time (60 to 180 seconds), and the pH of the buffer (2.0 to 8.0). The results indicate that the enzyme extraction conditions exhibited the least significant (P < 0.05) effect on temperature stability. Conversely, the extraction conditions had the most significant (P < 0.05) effect on the specific activity and pH stability. The results also reveal that the main effect of the B/S ratio, followed by its interaction with the pH of the buffer, was significant (P < 0.05) among most of the response variables studied. The optimum extraction condition caused the amylase to achieve high enzyme activity (648.4 U), specific activity (14.2 U/mg), temperature stability (88.4%), pH stability (85.2%), surfactant agent stability (87.2%), and storage stability (90.3%).

Item Type: Article
DOI Number: 10.1155/2014/640949
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1155/2014/640949
Keywords: Processing parameters; Amylase enzyme; Dragon fruit; Hylocereus polyrhizus; Response surface methodology
Divisions: Faculty of Food Science and Technology
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 23 Dec 2015 09:34
Last Modified: 23 Dec 2016 16:00
URI: http://psasir.upm.edu.my/id/eprint/34784

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