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Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles


Citation

Abdul Rahman, Samsulida and Saadun, Rafidah and Azmi, Nur Ellina and Ariffin, Nurhayati and Abdullah, Jaafar and Yusof, Nor Azah and Sidek, Hamidah and Hajian, Reza (2014) Label-free dengue detection utilizing PNA/DNA hybridization based on the aggregation process of unmodified gold nanoparticles. Journal of Nanomaterials, 2014. art. no. 839286. pp. 1-5. ISSN 1687-4110; ESSN: 1687-4129

Abstract / Synopsis

A label-free optical detection method based on PNA/DNA hybridization using unmodified gold nanoparticles (AuNPs) for dengue virus detection has been successfully developed. In this study, no immobilization method is involved and the hybridization of PNA/DNA occurs directly in solution. Unmodified AuNPs undergo immediate aggregation in the presence of neutral charge peptide nucleic acid (PNA) due to the coating of PNA on AuNPs surface. However, in the presence of complementary targets DNA, the hybridization of PNA probe with target DNA forms negatively charged complexes due to the negatively charged phosphate backbone of the target DNA. The negatively charged complexes adsorbed onto the AuNPs surface ensure sufficient charge repulsion, need for AuNPs dispersion, and stability in solution. The detection procedure is a naked eye method based on immediate color changes and also through UV-vis adsorption spectra. The selectivity of the proposed method was studied successfully by single base mismatch and noncomplementary target DNA.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
DOI Number: 10.1155/2014/839286
Publisher: Hindawi Publishing Corporation
Keywords: Label-free dengue detection; PNA; DNA; Hybridization; Aggregation process; Unmodified gold nanoparticles
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 10 Dec 2015 14:41
Last Modified: 15 Feb 2016 16:29
URI: http://psasir.upm.edu.my/id/eprint/34341
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