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Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus

Mohamed Shariff Mohamed Din. (2013) Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus.

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Abstract

The present invention provides a loop-mediated isothermal amplification (LAMP) method for the detection of infectious spleen and kidney necrosis virus (ISKNV) infection by amplifying a portion of ISKNV major capsid protein (MCP) gene using isothermal amplification and detecting the amplified product by acridine orange. Newly designed primers for use with this specific method are provided as well.The LAMP assay of the invention is specific towards ISKNV and there was no cross-amplification with other pathogens including the RSIV.

Item Type:Patent
Keyword:Loop-mediated isothermal amplification (LAMP); Infectious spleen; Kidney necrosis virus (ISKNV)
Faculty or Institute:Institute of Bioscience
Application Number:PI2013702063
Filing Date:2013-10-29
ID Code:33752
Deposited By: Mohd Hafiz Che Mahasan
Deposited On:31 Mar 2015 09:01
Last Modified:31 Mar 2015 09:01

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