Preliminary Study on the Production of Extracellular Protease from a Newly Isolated Bacillus sp. (No.1) and the Physical Factors Affecting Its Production

Abdul Razak, Che Nyonya and Tang, S. W. and Basri, Mahiran and Salleh, Abu Bakar (1997) Preliminary Study on the Production of Extracellular Protease from a Newly Isolated Bacillus sp. (No.1) and the Physical Factors Affecting Its Production. Pertanika Journal of Science & Technology, 5 (2). pp. 169-177. ISSN 0128-7680

[img] PDF
2276Kb

Abstract

A thermophilic bacterium isolated from a dumping ground in Sri Petaling, Kuala Lumpur, identified as Bacillus sp. (No.1), was able to grow at 600C and showed proteolytic activity on skim milk agar. The protease production by this bacterium and some physical factors affecting its production were investigated. Significant protease activity was detected in the basal medium after incubation for 16 h at 50°C with shaking at 200 rpm and reached its peak after 30 h. The protease production coincided with the late exponential or early stationary phase of bacterial growth and corresponded with the sporulation of this bacterium. The optimum pH, agitation rate and temperature for protease production by Bacillus sp. (No.1) were pH 8, 150 rpm and 50°C, respectively.

Item Type:Article
Keyword:extracellular protease, production, physical factors, Bacillus sp.
Publisher:Universiti Putra Malaysia Press
ID Code:3368
Deposited By: Nur Izzati Mohd Zaki
Deposited On:25 Nov 2009 07:34
Last Modified:27 May 2013 07:07

Repository Staff Only: item control page


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.