Early detection of Ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes
Siti Nor Akmar Abdullah. (2011) Early detection of Ganoderma boninense infection in oil palm based on expression of fatty acid biosynthesis pathway genes.
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The present invention relates to a method for early detection of Ganoderma boninense infection in oil palm and a method for indicating protection from Ganoderma boninense by a bio-control agent in a palm based on expression profile of genes associated with fatty acid (FA) biosynthetic pathway using roots and vegetative tissues comprising the step of: collecting a root or vegetative tissue sample, grounding the root or vegetative tissue sample and obtaining a powder, extracting the powder and obtaining a ribonucleic acid (RNA), designing at least a pair of primer based on the RNA, amplifying the pair of primer and obtaining an amplified nucleic acid and quantifying the amplified nucleic acid using a quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and obtaining a gene expression profile transcript characterize in that the RNA is from a de novo fatty acid biosynthetic gene protein coding region.
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