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Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions

Rashid Ibrahim. (2010) Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions.

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Abstract

The present invention provides a method of in vitro cultivation of mesenchymal stem cells into osteoblasts, in which total bone marrow taken from a mammal is cultivated in a complete medium containing an increased amount of fetal bovine serum under standard conditions, subsequently the non-adherent cells are removed and the adherent cells are further expanded in a complete medium containing an increased amount of fetal bovine serum and after the expansion they are cultivated in an osteogenic inducing medium containing an increased amount of fetal bovine serum under standard conditions. The method is suitable also for bone marrow taken from a health-compromised mammal.

Item Type:Patent
Keyword:Vitro isolation; Mesenchymal stem cells
Faculty or Institute:Faculty of Veterinary Medicine
Application Number:PI2010700063
Filing Date:2010-09-08
ID Code:32141
Deposited By: Junaini Mohd Yusof
Deposited On:03 Oct 2014 10:51
Last Modified:21 Nov 2016 12:15

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