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Method for the purification of interferon.

Ling Tau Chuan. (2011) Method for the purification of interferon.

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Abstract

The present invention discloses a method for the purification of a recombinant microbial Interferon α-2b in an aqueous two-phase system comprising a combination polymer and salt solution. In an embodiment of the present invention, the aqueous two-phase system comprising a combination of polymer and salt solution includes polyethylene glycol (PEG)-potassium phosphate, polyethylene glycol (PEG)-ammonium sulfate, polyethylene glycol (PEG)-sodium carbonate, polyethylene glycol (PEG)-sodium citrate and polyethylene glycol (PEG)-magnesium sulfate. The preferred aqueous two-phase system is the polyethylene glycol (PEG)-potassium phosphate system due to the benefits of low cost and short phase separation time as compared to other systems.

Item Type:Patent
Keyword:Purification; Polyethylene glycol; Interferon.
Faculty or Institute:Faculty of Engineering
Application Number:PI2011000150
Filing Date:2011-01-12
ID Code:32048
Deposited By: Suzila Mohamad Kasim
Deposited On:04 Sep 2014 12:38
Last Modified:04 Sep 2014 12:38

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