A method for recovering an intracellular Polyhydroxyalkanoates (PHA).
Mohd Ali Hassan. (2010) A method for recovering an intracellular Polyhydroxyalkanoates (PHA).
Full text not available from this repository.
The present invention relates to a method to recover an intracellular polyhydroxyalkanoates (PHA). The method includes the steps of (a) separating culture medium containing PHA-rich cells using centrifugation, (b) rinsing pellet obtained from setp (a) with distilled water, (c) freeze-drying pellet obtained in step (b), (d) adding distilled water to the freeze-dried sample, (e) separating non-PHA cellular material (NPCM)from PHA granules, (f) washing the pellet containing PHA from step (e) by distilled water and centrifuging for producing final pellet, and (g) freeze-drying the pellet produced from step (f).
Repository Staff Only: Edit item detail