Cryopreservation of Coffea liberica Seeds and Embryos Following Desiccation and Freezing Treatments

Y. L., Hor and P. C., Stanwood and H. F., Chin (1993) Cryopreservation of Coffea liberica Seeds and Embryos Following Desiccation and Freezing Treatments. Pertanika Journal of Tropical Agricultural Science, 16 (2). pp. 75-80. ISSN 0126-6128

[img] PDF
3012Kb

Abstract

Desiccation of Coffea liberica seeds for 6 days in an air-conditioned room reduced seed and attached emltryo moisture from 52.58% and 47.49% to 14.58% and 12.56% respectively. Seed germination and viability of the attached emltryo were maintained at moderate levels of 66% and 38% respectively. However, none of the desiccated seeds or embryos survived freezing in liquid nitrogen. Excised emltryos desiccated in the lamina flow cabinet lost their moisture very rapidly from 36.8% to 9.27% within 1.5 hours. More than 70% of these emltryos survived the desiccation. Moreover, partially desiccated emltryos at 17.17% moisture or less survived subfreezing temperatures. Between 35% to 50% survived slow freezing to -3ffC, but this was reduced to approximately 30% when they were subsequently plunged into liquid nitrogen. Fast freezing by direct plunge into liquid nitrogen also resulted in 10% to 35% survival. Desiccation ofexcised coffee embryos for 0.5h to 17.17% moisture was optimal for eryopreservation, irrespective of the speed offreezing. Differential thermal analyses of seed tissues suggest that the absence of freezable water is an important factor for successful eryopreservation of excised coffee emltryos. However, the importance of initial vigour, moisture variation and recovery media is also discussed.

Item Type:Article
Keyword:Cryopreservation, Coffea liberica, desiccation, freezing treatments
Publisher:Universiti Putra Malaysia Press
ID Code:3098
Deposited By: Nasirah Abu Samah
Deposited On:24 Nov 2009 01:25
Last Modified:27 May 2013 07:05

Repository Staff Only: item control page

Document Download Statistics

This item has been downloaded for since 24 Nov 2009 01:25.

View statistics for "Cryopreservation of Coffea liberica Seeds and Embryos Following Desiccation and Freezing Treatments"


Universiti Putra Malaysia Institutional Repository

Universiti Putra Malaysia Institutional Repository is an on-line digital archive that serves as a central collection and storage of scientific information and research at the Universiti Putra Malaysia.

Currently, the collections deposited in the IR consists of Master and PhD theses, Master and PhD Project Report, Journal Articles, Journal Bulletins, Conference Papers, UPM News, Newspaper Cuttings, Patents and Inaugural Lectures.

As the policy of the university does not permit users to view thesis in full text, access is only given to the first 24 pages only.