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Anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants.

Amin, Muhammad and Anwar, Farooq and Naz, Fauqia and Mehmood, Tahir and Saari, Nazamid (2013) Anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants. Molecules, 18 (2). pp. 2135-2149. ISSN 1420-3049

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Different parts of Acacia nilotica (L.) Delile, Calotropis procera (Aiton) W.T. Aiton, Adhatoda vasica Nees, Fagoniaar abica L. and Casuarina equisetifolia L. are traditionally used in folk medicine for the treatment of a variety of common ailments like nausea, cold, cough, asthma, fevers, diarrhea, sore throat, swelling, etc. The present study was aimed to evaluate the anti-Helicobacter pylori and urease inhibition activities of extracts produced from the above selected medicinal plants native to Soon Valley (home to an old civilization) in the Punjab province of Pakistan. Methanol, acetone and water extracts of the plants were evaluated for anti-bacterial activity against thirty four clinical isolates and two reference strains of H. pylori. Minimum inhibitory concentrations (MICs) of the extracts were determined using the agar dilution method and compared with some standard antibiotics like amoxicillin (AMX), clarithromycin (CLA), tetracycline (TET) and metronidazole (MNZ), used in the triple therapy for H. pylori eradication. H. pylori urease inhibition activity of the extracts was assessed by the phenol red method, wherein, Lineweaver-Burk plots were used to determine Michaelis-Menten constants for elucidating the mechanism of inhibition. Methanol and acetone extracts from Acacia nilotica and Calotropis procera exhibited stronger anti-H. pylori activity than MNZ, almost comparable activity with TET, but were found to be less potent than AMX and CLT. The rest of the extracts exhibited lower activity than the standard antibiotics used in this study. In the H. pylori urease inhibitory assay, methanol and acetone extracts of Acacia nilotica and Calotropis procera showed significant inhibition. Lineweaver-Burk plots indicated a competitive mechanism for extract of Acacia nilotica, whereas extract of Calotropis procera exhibited a mixed type of inhibition.

Item Type:Article
Keyword:Medicinal plants; Anti-Helicobacter pylori activity; Agar dilution method; Urease inhibitory activity; Lineweaver-Burk plots; Inhibition kinetics
Faculty or Institute:Faculty of Food Science and Technology
DOI Number:10.3390/molecules18022135
ID Code:30503
Deposited By: Khairil Ridzuan Khahirullah
Deposited On:03 Dec 2014 15:18
Last Modified:08 Sep 2015 11:35

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