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Anti- and pro-lipase activity of selected medicinal, herbal and aquatic plants, and structure elucidation of an anti-lipase compound

Ado, Muhammad Abubakar and Abas, Faridah and Mohammed, Abdulkarim Sabo and Mohd Ghazali, Hasanah (2013) Anti- and pro-lipase activity of selected medicinal, herbal and aquatic plants, and structure elucidation of an anti-lipase compound. Molecules, 18 (12). pp. 14651-14669. ISSN 1420-3049; ESSN: 1420-3049

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Official URL: http://www.mdpi.com/1420-3049/18/12/14651

Abstract

Plants that help in slowing down the digestion of triacylglycerols (TAGs) in the pancreas and small intestine of humans play an important role in the reduction of obesity. On the other hand, there may be plants or plant parts that stimulate intestinal lipolytic activity, thus contributing to greater TAG assimilation. The aim of this study was to evaluate the aqueous methanolic extracts of ninety eight (98) medicinal, herbal and aquatic plant materials from Malaysia for their effect on porcine pancreatic lipase (PPL) activity and to identify the structure of an anti-lipase compound from one of the sources. The degree of inhibition was also quantified as relative to orlistat activity against PPL (orlistat equivalents). Results revealed that while 19.4% of the extracts were found to have anti-lipase activity ≥80%, 12% were actually found to promote PPL activity. Twenty two percent (22.4%) exhibited moderate inhibition (41%–80%) and 2% were neutral toward PPL activity. The ripe fruit of Averrhoa carambola and the leaves of Archidendron jiringa (Jack) I.C Nielsen L. (jering), Cynometra cauliflora (nam-nam) and Aleurites moluccana (L.) Willd (candle nut/buah keras) had the highest (100%) anti-lipase activity and are equivalent to 0.11 µg orlistat/mL. Plants that stimulated lipase activity included Pimpinella anisum L. (aniseed/jintan manis), activating the enzyme by 186.5%. Kaempferol 3-O-rhamnoside was isolated from the ethyl acetate fraction of C. cauliflora leaves and found to be an active lipase inhibitor. The structure was elucidated using 1H-NMR, 13C-NMR and 2D-NMR analyses.

Item Type:Article
Keyword:Anti-lipase activity; Pro-lipase activity; Plant extracts; Orlistat equivalent; Kaempferol 3-O-rhamnoside
Faculty or Institute:Faculty of Food Science and Technology
Institute of Bioscience
Publisher:MDPI
DOI Number:10.3390/molecules181214651
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules181214651
ID Code:30479
Deposited By: Khairil Ridzuan Khahirullah
Deposited On:03 Dec 2014 10:48
Last Modified:08 Sep 2015 11:32

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