Suparman, Maman (2004) Antagonistic Bacteria for Controlling Fusarium Wilt of Tomato Caused by Fusarium Oxysporum F. Sp. Lycopersici. Masters thesis, Universiti Putra Malaysia.
Four bacterial isolates, namely Serratia marcescens, Burkholderia cepacia, Pseudomonas aeruginosa 1 and P. aeruginosa 2 obtained from the rhizosphere of tomato plants in the field which were antagonistic to the growth of F. oxysporum f.sp. lycopersici in vitro, were evaluated for their ability to suppress Fusarium wilt disease of tomato in the glasshouse. The tomato plants were planted in growth media consisting of Holland RHP® peat grow, coconut peat and perlite in the ratio of 1:1:1(v/v). Tomato plants were watered using the drip irrigation system for distribution of hydroponics fertilizer solution. Repeated applications of 10 mL of bacterial suspensions were applied at 10 days and 20 days after sowing at a concentration of 1x10 8 cfu mL-1 Plants inoculated with all four antagonistic bacteria, individually and in mixture showed increased plant growth. Application of these bacteria significantly increased fresh weight, dry weight, plant height and initial flowering of tomato plants. B. cepacia and P. aeruginosa 1 were found to suppress disease severity up to 100%, both at seedling stage and standing crops.Plants treated with mixture of B. cepacia, P. aeruginosa 1 and P.aeruginosa 2 significantly increased the marketable fruit yield in artificially infected plants compared to the control, although there was not significantly difference as between single treatments of the four bacterial isolates.The experiment carried out in this study indicated that the four antagonistic bacteria functioned as plant growth promoters as well as biological control agents for Fusarium wilt of tomato.
|Item Type:||Thesis (Masters)|
|Subject:||Fusarium wilt of tomato - Fusarium oxysporum - Case studies|
|Chairman Supervisor:||Associate Professor Kamaruzaman Sijam, PhD|
|Call Number:||FP 2004 19|
|Faculty or Institute:||Faculty of Agriculture|
|Deposited By:||INVALID USER|
|Deposited On:||06 Jun 2008 00:16|
|Last Modified:||03 Apr 2012 10:33|
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