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Characterization of a probiotic Lactobacillus fermentum isolated from snakehead, channa striatus, stomach

Allameh, Sayed K. and Md. Yusoff, Fatimah and Mohd Daud, Hassan and Ringo, Einar and Ideris, Aini and Saad, Che Roos (2013) Characterization of a probiotic Lactobacillus fermentum isolated from snakehead, channa striatus, stomach. Journal of the World Aquaculture Society, 44 (6). pp. 835-844. ISSN 0893-8849; ESSN: 1749-7345

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Abstract

This study sought to isolate and characterize lactic acid bacteria (LAB) from stomach of adult snakehead fish, Channa striatus, to be used as probiotics for freshwater fish. A total of 13 strains were isolated from the stomach of 10 fish, and 4 of these belonged to LAB. Strain LAB-3 showing highest in vitro growth inhibition of Aeromonas hydrophila in a disk diffusion test was identified as Lactobacillus fermentum by conventional and molecular techniques and evaluated in vitro through various tests. The bacterium could grow at pH 3–8; but the optimum growth was observed at pH 6. Moreover, LAB-3 grew at 0.15 and 0.3% bile salt concentrations, from 15 to 45 C, and at 4% NaCl. L. fermentum showed in vitro inhibitory activity against three fish pathogens, A. hydrophila, Pseudomonas aeruginosa, and Shewanella putrefaciens, tested by disk diffusion and well diffusion methods. Antibiotic sensitivity tests indicated that L. fermentum was resistant to streptomycin, gentamycin, and kanamycin, intermediate to tetracycline, but sensitive to chloramphenicol, amoxicillin, and ampicillin. Challenge test by using A. hydrophila showed that survival of snakehead was significantly (P < 0.05) improved when 2 × 106 LAB-3/g was supplemented to the diet. Therefore, this study suggests that L. fermentum might be a promising probiotic in snakehead aquaculture.

Item Type:Article
Keyword:Probiotic; Lactobacillus fermentum; Lactic acid bacteria; Snakehead fish; Channa striatus.
Faculty or Institute:Faculty of Veterinary Medicine
Faculty of Agriculture
Institute of Bioscience
Publisher:Wiley-Blackwell
DOI Number:10.1111/jwas.12075
Altmetrics:http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/jwas.12075
ID Code:29916
Deposited By: Azizan Arshad
Deposited On:09 Oct 2014 15:05
Last Modified:08 Sep 2015 15:02

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