Keyword Search:


Bookmark and Share

Clinico-pathological changes associated with Brucella melitensis infection and its bacterial Lipopolysaccharides (LPS) in male mice

Abdullah, Faez Firdaus Jesse and Nik, Norasiah and Saad, Mohd Zamri and Haron, Abd Wahid and Omar, Abdul Rahman and Sabri, Jasni and Adamu, Lawan and Osman, Abdinasir Yusuf and Saharee, Abdul Aziz (2013) Clinico-pathological changes associated with Brucella melitensis infection and its bacterial Lipopolysaccharides (LPS) in male mice. International Journal of Animal and Veterinary Advances, 5 (5). pp. 165-170. ISSN 2041-2894; ESSN: 2041-2908

[img] PDF (Abstract)
180Kb

Official URL: http://www.maxwellsci.com/jp/abstract.php?jid=IJAV...

Abstract

Brucella melitensis (B. melitensis) is gram negative, aerobic bacteria that cause Brucellosis in humans’ sheep and goats. Brucellosis causes abortion in wild and domestic animals resulting in enormous financial losses. Therefore, the purpose of this study was to evaluate the clinico-pathological changes associated with Brucella melitensis infection and its bacterial Lipopolysaccharides (LPS) in male mice. Three groups of 24 Balb/c male mice consisting of 8 mice in each group were used as an animal model for the study. The control group were inoculated intraperitoneally with 1 mL of Phosphate Buffered Solution (PBS) pH 7 while, the treatment groups were inoculated intraperitoneally with 1 mL×109 of B. melitensis colony and 1 mL×109 of of Lipopolysaccharides (LPS) extracted from B. melitensis respectively. Mice that showed severe clinical signs and those that survived were euthanized by cervical dislocation method after 5 days of post infection subsequently, post mortem was conducted and histopathological studies were carried out. B. melitensis group showed severe clinical signs between 6 to 17 h of post inoculation compared to the PBS and LPS groups. The LPS group became lethargic 2 h post inoculation but, they become active after 5 h post inoculation, while the control group (PBS) exhibited normal responses. Histopathology results showed severe tissue alterations in the reproductive organs of the B. melitensis group compared to LPS group. In conclusion, the atrophy of the spermatocytes in the testes and degenerative necrosis of the pseudo stratified epithelium of the vas deferens in the B. melitensis group were severe while, LPS group showed moderate atrophy of the spermatocyte of the testes and severe degenerative necrosis of the pseudo stratified epithelium of the vas deferens.

Item Type:Article
Keyword:B. melitensis; Brucellosis; Lipopolysaccharides; Spermatocyte; Vas deferens
Faculty or Institute:Faculty of Veterinary Medicine
Institute of Bioscience
Publisher:Maxwell Science Publication
ID Code:29856
Deposited By: Nabilah Mustapa
Deposited On:05 May 2015 10:17
Last Modified:08 Sep 2015 15:30

Repository Staff Only: Edit item detail